Tuesday, December 23, 2008
Viral Amplification
What is Viral Amplification???
Viral amplification is used to infect the cells and wait for multiple rounds of infection to grow the virus in large quantities. It is a useful method to get satisfying concentration of virus and to conduct further studies on the virus amplified.
The virus is first cultivated in cells in a small flask, the medium is then used to infect a larger flask to produces higher titres. To amplify the virus, the virus needed to be cultivated in a continuous cell line rather than primary cell line because primary cell line have a limited life span and needed to be regenerated every time. The multiplicity of infection (MOI), the ratio of infectious virus to infection cells must be at least 10 to get a better yield of virus.
The virus is first cultivated in cells in a small flask, the medium is then used to infect a larger flask to produces higher titres. To amplify the virus, the virus needed to be cultivated in a continuous cell line rather than primary cell line because primary cell line have a limited life span and needed to be regenerated every time. The multiplicity of infection (MOI), the ratio of infectious virus to infection cells must be at least 10 to get a better yield of virus.
Typical Steps to conduct Viral Amplication
Results
After viral amplification, the infected Vero cells which are high in titre do not divide but increasing in size and appear granular under microscope. However, the infected Vero cells which do not undergo viral amplification are low in titre, hence they continue to grow to confluency
Blogged @ 6:54 AM