Tuesday, January 6, 2009
ELISA
ELISA - Enzyme-Linked ImmunoSorbent Assay
What is ELISA???
ELISA is a technique to detect the presence or a particular antigen and antibody in samples. It is commonly use in labs to determine diseases in humans, animals and plants.
The basic principle of ELISA is to use an enzyme to detect the binding of antigen and antibodies. The enzyme convert the colourless nsubstrate to a coloured product indication the antigen antibodies binding.
The ELISA experiment can be designed in many way to see if you are detecting antibodies or antigen. It is also one of the easier way to determine the presence of a particular antigen or antibodies.
What are the advantages of ELISA?
ELISA tests are generally relatively accurate tests. They are considered highly sensitive and specific and compare favorably with other methods used to detect substances in the body, such as radioimmune assay (RIA) tests. They have the added advantages of not needing radioisotopes (radioactive substances) or a costly radiation counter (a radiation-counting apparatus).
ELISA EXPERIMENT Materials
ELISA is a technique to detect the presence or a particular antigen and antibody in samples. It is commonly use in labs to determine diseases in humans, animals and plants.
The basic principle of ELISA is to use an enzyme to detect the binding of antigen and antibodies. The enzyme convert the colourless nsubstrate to a coloured product indication the antigen antibodies binding.
The ELISA experiment can be designed in many way to see if you are detecting antibodies or antigen. It is also one of the easier way to determine the presence of a particular antigen or antibodies.
What are the advantages of ELISA?
ELISA tests are generally relatively accurate tests. They are considered highly sensitive and specific and compare favorably with other methods used to detect substances in the body, such as radioimmune assay (RIA) tests. They have the added advantages of not needing radioisotopes (radioactive substances) or a costly radiation counter (a radiation-counting apparatus).
ELISA EXPERIMENT Materials
- Distilled water
- 37 C incubator
- Measuring Cylinder
- Micropippette
- Microplate reader
- Conjugate Solution
- Diluent buffer
- Coating Solution (10mM PBS pH 7.2)
- Blocking Solution (gelatin)
- Primary/Secondary AntibodySolution
- Wash Solution (PBS)
Blogged @ 6:24 AM